cjun (Cell Signaling Technology Inc)
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Cjun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cjun/c-Jun+Rabbit+mAb/pmc13032856-436-43-44
Average 96 stars, based on 1587 article reviews
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Incubation:Article Title: Palmitoylation licenses RIPK1 kinase activity and cytotoxicity in the TNF pathway. Article Snippet: Article Article Title: The inhibition of Il1β synthesis mediated by a novel pyridine-sulfonamide compound protects against the progression of metabolic dysfunction-associated steatotic liver disease. Article Snippet: .. After blocking, membranes were incubated overnight with primary antibodies at 4◦C: anti-IκBα (sc-371) and anti-JNK (sc7345) from Santa Cruz Biotechnology Inc.; anti-IL1β (AF-401-NA) from Bio-Techne R&D Systems; anti-cleaved caspase-3 (#9661), antiphospho-JNK (#4668), anti-phospho-p38 (#9211), anti-p38 (#9212), anti-phospho-cJun (#9164) and Article Title: Regional modulation of toll-like receptor signaling pathway genes in acute epididymitis in mice. Article Snippet: 1Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil 2Institute of Anatomy and Cell Biology, Unit of Reproductive Biology, Justus-Liebig-University Giessen, Giessen, Germany 3Department of Pharmacology, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, São Paulo, Brazil 4Hessian Center of ReproductiveMedicine, Justus-Liebig-University of Giessen, Giessen, Germany 5Centre of Reproductive Health, Hudson Institute ofMedical Research, Clayton, Australia Immunohistochemistry:Article Title: Pioglitazone modulates metabolic adaptation and peripheral nerve regeneration after injury Article Snippet: Sciatic nerves were fixed in 4% paraformaldehyde (PFA), embedded in paraffin and sectioned into 5 μm slices using a microtome (Leica). .. Immunohistochemistry was performed using the following primary antibodies: anti-PPARɣ (rabbit, 1:5000, Abcam, ab59256), anti-PLIN2/ADFP (rabbit, 1:500, Novus biologicals, NB110-40877), anti-CD45 (rat, 1:100, BD Pharmingen, 550539), and Blocking Assay:Article Title: The inhibition of Il1β synthesis mediated by a novel pyridine-sulfonamide compound protects against the progression of metabolic dysfunction-associated steatotic liver disease. Article Snippet: .. After blocking, membranes were incubated overnight with primary antibodies at 4◦C: anti-IκBα (sc-371) and anti-JNK (sc7345) from Santa Cruz Biotechnology Inc.; anti-IL1β (AF-401-NA) from Bio-Techne R&D Systems; anti-cleaved caspase-3 (#9661), antiphospho-JNK (#4668), anti-phospho-p38 (#9211), anti-p38 (#9212), anti-phospho-cJun (#9164) and Article Title: Regional modulation of toll-like receptor signaling pathway genes in acute epididymitis in mice. Article Snippet: 1Department of Biophysics and Pharmacology, Institute of Biosciences of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil 2Institute of Anatomy and Cell Biology, Unit of Reproductive Biology, Justus-Liebig-University Giessen, Giessen, Germany 3Department of Pharmacology, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, São Paulo, Brazil 4Hessian Center of ReproductiveMedicine, Justus-Liebig-University of Giessen, Giessen, Germany 5Centre of Reproductive Health, Hudson Institute ofMedical Research, Clayton, Australia |
![A subpopulation of RGCs express the stress marker phosphorylated c-JUN <t>(p-cJUN).</t> (A) Whole mount image ( Cx3cr1-Gfp/+ ) of e14.5 retina showing that a subset of RGCs (Brn3a+; red) express nuclear p-cJUN (white; yellow arrows). Boxed regions show enlarged region and label for p-cJUN alone or Brn3a alone (A′) . Scale bar = 10 μm. (B) Whole mount image (e14.5 Cx3cr1-Gfp/+ ) showing microglia (GFP) internalizing an RGC (Brn3a + red) labeled with p-cJUN (white) using HCR IHC. Scale bar = 1 μm. (C) Whole mount images showing RGCs (Brn3a+; red) with nuclear p-cJUN (white) in control mice (WT) and Mertk/CR3 dKO mice. Yellow arrows indicate double positive p-cJUN and Brn3a staining. (D) Quantification of double positive p-cJUN + Brn3a + RGCs per mm 2 in e14.5 control and Mertk/CR3 dKO mice. *** p < 0.01 unpaired t -test [ N = 4 (WT), 5 (Mertk CR3 dKO)]. (E) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in e14.5 and e16.5 Mertk/CR3 dKO mice. * p < 0.05 Welch’s t -test [ N = 5 (e14.5), 4 (e16.5)]. (F) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in P0 control, Mertk KO and Mertk/CR3 dKO mice. ns, not significant. Welch’s one-way ANOVA with Brown–Forsythe variance test [ N = 7 (WT), 7 (Mertk KO), 6 (Mertk CR3 dKO)]. (G) Whole mount retina images to show vehicle treated Axl KO mice with RGCs (Brn3a+; red) expressing nuclear p-cJUN (white) and microglia present (IBA1+; green). Scale bar = 500 μm. Boxed regions show enlarged area (G′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100-10 μm. (H) Whole mount retina images to show Axl KO PLX treated mice with depletion of microglia (IBA1; green) and RGCs (Brn3a+; red) with nuclear p-cJUN (white) expression remaining. Boxed regions show enlarged area (H′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100 to 10 μm.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5010/pmc12715010/pmc12715010__fncel-19-1719791-g002.jpg)